產(chǎn)品編號 yb-1145R
英文名稱 Anti-ARF6抗體
中文名稱 ADP核糖基化因子6抗體
別    名 ADP ribosylation factor 6; ADP ribosylation factor protein 6; ADP-ribosylation factor 6; Small GTPase; AI788669; AW496366; MGC102530; ADP ribosylation factor protein 6; ARF6; ARF6_HUMAN; DKFZp564M0264; Small GTP binding protein.
Anti-ARF6抗體   
說 明 書 0.1ml  0.2ml  
研究領(lǐng)域 信號轉(zhuǎn)導(dǎo)  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應(yīng) Human, Mouse, Rat, Chicken, Pig, Horse, Rabbit, 
產(chǎn)品應(yīng)用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蠟切片需做抗原修復(fù)） 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 20kDa
細(xì)胞定位 細(xì)胞漿 細(xì)胞膜 
性    狀 Lyophilized or Liquid
濃    度 1mg/1ml
免 疫 原 KLH conjugated synthetic peptide derived from human ARF6
亞    型 IgG
純化方法 affinity purified by Protein A
儲 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

PubMed PubMed
產(chǎn)品介紹 background:
This gene encodes a member of the human ARF gene family, which is part of the RAS superfamily. The ARF genes encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The product of this gene is localized to the plasma membrane, and regulates vesicular trafficking, remodelling of membrane lipids, and signaling pathways that lead to actin remodeling. A pseudogene of this gene is located on chromosome 7.

Function:
GTP-binding protein involved in protein trafficking; regulates endocytic recycling and cytoskeleton remodeling. May modulate vesicle budding and uncoating within the Golgi apparatus. Functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Involved in the regulation of dendritic spine development. Contributes to the regulation of dendritic branching and filopodia extension.

Subunit:
Interacts with ARHGAP21, ASAP2, HERC1, PIP5K1C and UACA. Interacts with NCS1/FREQ at the plasma membrane. Interacts with RAB11FIP3 and RAB11FIP4. Interacts with USP6 (via Rab-GAP TBC domain). Interacts with ECM29. Interacts with TBC1D24.

Subcellular Location:
Golgi apparatus. Cell membrane; Lipid-anchor. Endosome membrane; Lipid-anchor. Cell membrane. Cell projection, filopodium membrane; Lipid-anchor. Note=Recruited to the cell membrane in association with CYTH2 and ARL4C.

Similarity:
Belongs to the small GTPase superfamily. Arf family.

主要用于：腫瘤方面的研究，在很多腫瘤組織中都有一定的表達(dá)及分布。
技術(shù)背景：rRNA 加工、內(nèi)質(zhì)網(wǎng)高爾基膜泡介導(dǎo)運(yùn)輸、細(xì)胞運(yùn)動性、細(xì)胞附著、小GTP 酶介導(dǎo)信號轉(zhuǎn)導(dǎo)、蛋白運(yùn)輸、膜泡介導(dǎo)運(yùn)輸、正調(diào)控肌動蛋白絲聚合、皮層肌動蛋白細(xì)胞骨架組織和生物發(fā)生、褶邊組織和生物發(fā)生、調(diào)控Rac 蛋白信號轉(zhuǎn)導(dǎo)、負(fù)調(diào)控受體介導(dǎo)胞吞.